Methods & Reagents

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and ______ sections are pretreated _______. For immunodetection, nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. Sections are incubated in primary antibody (_______) _______. After rinsing, sections are incubated for _______ in biotinylated secondary antibody, rinsed, incubated for _______ in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, alpha-synuclein sections are pretreated with 90% formic acid for 10 min at room temperature. Sections are incubated in primary antibody (1:20000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, β-amyloid sections are pretreated with 90% formic acid for 10 min at room temperature. Sections are incubated in primary antibody (1:5000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, FUS sections are pretreated microwave in 0.1M citrate buffer 2x2.5 min. Sections are incubated in primary antibody (1:250) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, GFAP (mouse monoclonal antibody) sections are pretreated (no pretreatment). Sections are incubated in primary antibody (1:100) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, GFAP (rabbit polyclonal antibody) sections are pretreated (no pretreatment). Sections are incubated in primary antibody (1:500) 30 min at 40°C. After rinsing, sections are incubated for 15 min at 40°C in biotinylated secondary antibody, rinsed, incubated for 30 min at 40°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, neurofilaments sections are pretreated with pepsin for 10 min at 40°C. Sections are incubated in primary antibody (1:100) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, tau sections are pretreated (microwave in 0.1M citrate buffer 2x2.5 min). Sections are incubated in primary antibody (1:1000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 370°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 370°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, TDP-43 sections are pretreated microwave in 0.1M citrate buffer 2x2.5 min. Sections are incubated in primary antibody (1:500) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, phospho-TDP-43 sections are pretreated microwave in 0.1M citrate buffer 2x2.5 min. Sections are incubated in primary antibody (1:8000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, tyrosine hydroxylase sections are pretreated microwave in 0.1M citrate buffer 2x2.5 min. Sections are incubated in primary antibody (1:1000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 37°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 37°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.

Endogenous perox idase in tissue sections is blocked with 3% H2O2 in methanol for 5 min at 40°C, and nonspecific reagent binding is blocked with normal serum in 0.1 M Tris each for one hour at room temperature. For immunodetection, ubiquitin sections are pretreated (no pretreatment). Sections are incubated in primary antibody (1:1000 or 1:2000) overnight at 4°C. After rinsing, sections are incubated for 30 min at 370°C in biotinylated secondary antibody, rinsed, incubated for 60 min at 370°C in avidin-biotin complex, and then developed with diaminobenzidine (DAB). Appropriate positive controls are included in each immunohistochemical experiment. Negative controls consist of sections incubated without primary antibody.